Entry retrieved from the Protein Data
Bank at RCSB.
1REQ: Isomerase. Date: Jan 19, 1996.
Title: Methylmalonyl-CoA Mutase
Authors: F.Mancia, N.H.Keep, A.Nakagawa, P.F.Leadlay,
S.Mcsweeney, B.Rasmussen, P.Bosecke, O.Diat, P.R.Evans
Compound: Methylmalonyl-Coa Mutase
Ec: 5.4.99.2 Desulpho-Coa, And A Glycerol In The Active Site. B12 Is
Present Largely As Reduced Cob(II)Alamin, Or B12r.
Source: Propionibacterium Freudenreichii Subsp. Shermanii.
Method: X-Ray Diffraction. Resolution 2.0 Ang.
Ligands: B12 cobalamin, DCA (desulfo coenzyme A), GOL (glycerol).
Methylmalonyl-CoA mutase catalyzes the isomerization of
methylmalonyl-CoA into succinyl-CoA
Me-CH(COO-)C(O)-S-CoA into (-OOC)CH2-CH2-C(O)-S-CoA
The isomerase is displayed as "ribbons" and the color
is selected so as to show the "chain". The coenyzme
B12 is shown in
CPK color and in spacefill mode.
Note the presence of glycerol molecules and of desulfo coenzyme A.
The next panel shows the coenzyme, DCA and the metal co-factor alone.