Prelab 3 SUBTOTAL PRE-LAB: 3 points THIN-LAYER CHROMATOGRAPHY Drawing of TLC plate 1 Determine 3 distances in mm (solvent, 2 comp.) 1 Compute two Rf values 1 COLUMN CHROMATOGRAPHY Recording of the amount of tomato paste used 1 Recording of the volume of the lycopene fraction 1 Absorbance of the lycopene fraction 1 UV-spectrum of lycopene (web) 1 Drawing of TLC plate, compute & record Rf values 1 Successful separation 1 Initials in Lab Book after end of session 2 SUBTOTAL LAB: 11 points Evaluation of Column Chromatography Calc. amount of lycopene (moles, mg) 1 Calc. weight percentage of lycopene 1 Post-Lab Questions 4 SUBTOTAL POST-LAB: 6 points