Prelab 3
SUBTOTAL PRE-LAB: 3 points
THIN-LAYER CHROMATOGRAPHY
Drawing of TLC plate 1
Determine 3 distances in mm (solvent, 2 comp.) 1
Compute two Rf values 1
COLUMN CHROMATOGRAPHY
Recording of the amount of tomato paste used 1
Recording of the volume of the lycopene fraction 1
Absorbance of the lycopene fraction 1
UV-spectrum of lycopene (web) 1
Drawing of TLC plate, compute & record Rf values 1
Successful separation 1
Initials in Lab Book after end of session 2
SUBTOTAL LAB: 11 points
Evaluation of Column Chromatography
Calc. amount of lycopene (moles, mg) 1
Calc. weight percentage of lycopene 1
Post-Lab Questions 4
SUBTOTAL POST-LAB: 6 points