MU Chemistry 416 FS97

Applications of UV/Vis Spectroscopy

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Visible Spectra of Conjugated Dyes: Integrating Quantum Chemical Concepts with Experimental Data. George M. Shalhoub J. Chem. Educ. 1997, 74, 1317-1319.

Application of the particle in a box theory to a series of dyes. Read this to learn about the integrated absorption coefficient (IAC). IAC is the peak area in a plot of the extinction coefficient as a function of the frequency. IAC values are a better measure of the transition moment than are the absorptivities. The Einstein coefficient, the transition moment, and the oscillator strength all follow directly from the IAC value.

C60 and C70 in a Basket? - Investigations of Mono- and Multilayers from Azacrown Compounds and Fullerenes. Ringsdorf, H. et al. Angew. Chem. Int. Ed. Engl. 1992, 31, 1599-1602. UV-Topic: Aggregation study using UV/Vis Chem Topic: Langmuir-Blodgett (LB) films

This paper demonstrates the use of UV/Vis-spectroscopy in studies of aggregation and in the formation of Langmuir-Blodgett (LB) films. (Summary Sheet)

(a) Transferrin: The Role of Conformational Change in Iron Removal by Chelators. Raymond, K. N. et al. J. Am. Chem. Soc. 1993, 115, 6758-6764. (b) Lactoferrin: The Role of Conformational Changes in Its Iron Binding and Release. Raymond, K. N. et al. J. Am. Chem. Soc. 1993, 115, 6765-6768.

These two papers demonstrate well the use of UV/Vis spectroscopy to study kinetics. Derivative spectroscopy also is employed. The chemical topic of these papers concerns the elucidation of metal binding in enzymes, a topic that has enjoyed increasing attention since the late 80's. (Summary Sheet)

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